General Cell Based Assays
Eurofins Discoverx PathHunter eXpress MRGPRX4 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0541E2ACP1M)
The PathHunter MRGPRX4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of MRGPRX4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MRGPRX4 and EA-tagged β-Arrestin-2. Activation of MRGPRX4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCCR2 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0543E3MCP5M)
The PathHunter mCCR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL2/MCP-1) based activation of mCCR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR2 and EA-tagged β-Arrestin-2. Activation of mCCR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 30 Reagent, 500. (93-0563R30B)
AssayComplete Cell Plating 30 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Meso Scale Discovery U-PLEX Mouse TNF- ASY
The Meso Scale Discovery U-PLEX Mouse TNF-alpha Assay is a multiplex immunoassay kit that enables simultaneous measurement of mouse tumor necrosis factor alpha (TNF-alpha) in serum plasma and cell culture supernatants It utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision This assay requires 50 L or less sample volume and can be fully automated on the MSD instrument platforms The lower limit of detection is 0 04 pg/mL
Eurofins Discoverx HitHunter cGMP Assay, 2,400 dp (384-well). (90-0039M)
The HitHunter cGMP Assay is a detection kit offering a robust, sensitive and easy-to-use assay to measure intracellular cGMP levels. This product uses EFC technology, and contains ED-tagged cGMP, anti-cGMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cGMP, the anti-cGMP antibody will be sequestered by binding to this untagged cGMP, allowing for EFC between the tagged cGMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit may be used with Eurofins DiscoverX cell lines as well as cell lines from other sources. This detection kit contains reagents for 6 plates (384 well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
Meso Scale Discovery R-PLEX Human IL-34 ASY
The Meso Scale Discovery R-PLEX Human IL-34 Assay Kit is a multiplex immunoassay that measures IL-34 in human serum plasma and cell culture supernatants The assay uses electrochemiluminescence detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample volume requirements The kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with MSD SULFO-TAG calibrators and diluent The assay takes 3 5 hours to complete and is suitable for measuring natural and recombinant human IL-34
Eurofins Discoverx PathHunter eXpress IL17RA/IL17RC Dimerization Assay, 10pl Kit. (93-0999E3CP0L)
The PathHunter IL17RA/IL17RC (U2OS) Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-17a) induced dimerization of IL17RA/IL17RC (U2OS) receptors. This product uses EFC technology. The cell line included overexpresses IL17RC target protein fused to PK alongside IL17RA target protein fused to EA. Upon ligand-induced dimerization of IL17RA/IL17RC, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL4R/IL13RA1 Dimerization Assay, U2OS, 2pl Kit. (93-1000E3CP5M)
The PathHunter IL4R/IL13RA1 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-13) induced dimerization of IL4R/IL13RA1 receptors. This product uses EFC technology. The cell line included overexpresses IL4R target protein fused to PK alongside IL13RA1 target protein fused to EA. Upon ligand-induced dimerization of IL4R/IL13RA1, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mPTGER4 DLD1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0982E13MCP25M)
The PathHunter mPTGER4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of mPTGER4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mPTGER4 and EA-tagged β-Arrestin-2. Activation of mPTGER4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress cyno CXCR1 HEK 293 β-Arrestin GPCR Assay, 10pl Kit. (93-0980E1CCP0L)
The PathHunter cyno CXCR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL8/IL8) based activation of cyno CXCR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged cyno CXCR1 and EA-tagged β-Arrestin-2. Activation of cyno CXCR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Cynomolgus monkey version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IFNRL1/IL10RB Dimerization Assay, 2pl Kit. (93-0993E3CP5M)
The PathHunter IFNRL1/IL10RB Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-29) induced dimerization of IFNRL1/IL10RB receptors. This product uses EFC technology. The cell line included overexpresses IFNRL1 target protein fused to PK alongside IL10RB target protein fused to EA. Upon ligand-induced dimerization of IFNRL1/IL10RB, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress P2RY1 Activated GPCR Internalization Assay, 10pl Kit. (93-0967E3CP0L)
The PathHunter P2RY1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of P2RY1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged P2RY1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting P2RY1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter IL10RA/10RB Dimerization Bioassay Kit, 10pl. (93-0985Y3-00136)
The PathHunter IL10RA/IL10RB Dimerization Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-10) induced dimerization of IL10RA/IL10RB receptors. This product uses EFC technology. The cell line included overexpresses IL10RA target protein fused to PK alongside IL10RB target protein fused to EA. Upon ligand-induced dimerization of IL10RA/IL10RB, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress CCR6 Activated GPCR Internalization Assay, 10pl Kit. (93-0966E3CP5L)
The PathHunter CCR6 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCR6 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CCR6. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CCR6-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Multiplex Human & Mouse Phospho-SMAD2 (Ser465/467 & Total) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Multiplex Human & Mouse Phospho-SMAD2 (Ser465/467 & Total) Detection Kit, 500 Assay Points